Names & Taxonomy
- Uniprot ID:
- Q9UPY3
- Entry Name:
- DICER_HUMAN
- Status:
- reviewed
- Protein Names:
- Endoribonuclease Dicer (EC 3.1.26.3) (Helicase with RNase motif) (Helicase MOI)
- Gene Names:
- DICER1 DICER HERNA KIAA0928
- Gene Names Primary:
- DICER1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 1922
- Sequence:
- MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
- Catalytic Activity:
- Endonucleolytic cleavage to 5'-phosphomonoester.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Gene Ontology Go:
- ARC complex
axon
cytoplasm
cytosol
dendrite
endoplasmic reticulum-Golgi intermediate compartment
growth cone
nucleus
RISC-loading complex
ATP binding
deoxyribonuclease I activity
DNA binding
double-stranded RNA binding
endoribonuclease activity
helicase activity
metal ion binding
pre-miRNA binding
protein domain specific binding
ribonuclease III activity
siRNA binding
apoptotic DNA fragmentation
conversion of ds siRNA to ss siRNA
conversion of ds siRNA to ss siRNA involved in RNA interference
gene expression
gene silencing by RNA
miRNA loading onto RISC involved in gene silencing by miRNA
negative regulation of Schwann cell proliferation
negative regulation of transcription from RNA polymerase II promoter
nerve development
neuron projection morphogenesis
peripheral nervous system myelin formation
positive regulation of myelination
positive regulation of Schwann cell differentiation
pre-miRNA processing
production of miRNAs involved in gene silencing by miRNA
production of siRNA involved in RNA interference
RNA phosphodiester bond hydrolysis, endonucleolytic
siRNA loading onto RISC involved in RNA interference
targeting of mRNA for destruction involved in RNA interference - Gene Ontology Biological Process:
- apoptotic DNA fragmentation
conversion of ds siRNA to ss siRNA
conversion of ds siRNA to ss siRNA involved in RNA interference
gene expression
gene silencing by RNA
miRNA loading onto RISC involved in gene silencing by miRNA
negative regulation of Schwann cell proliferation
negative regulation of transcription from RNA polymerase II promoter
nerve development
neuron projection morphogenesis
peripheral nervous system myelin formation
positive regulation of myelination
positive regulation of Schwann cell differentiation
pre-miRNA processing
production of miRNAs involved in gene silencing by miRNA
production of siRNA involved in RNA interference
RNA phosphodiester bond hydrolysis, endonucleolytic
siRNA loading onto RISC involved in RNA interference
targeting of mRNA for destruction involved in RNA interference - Gene Ontology Molecular Function:
- ATP binding
deoxyribonuclease I activity
DNA binding
double-stranded RNA binding
endoribonuclease activity
helicase activity
metal ion binding
pre-miRNA binding
protein domain specific binding
ribonuclease III activity
siRNA binding - Gene Ontology Cellular Component:
- ARC complex
axon
cytoplasm
cytosol
dendrite
endoplasmic reticulum-Golgi intermediate compartment
growth cone
nucleus
RISC-loading complex - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Complete proteome
Cytoplasm
Disease mutation
Endonuclease
Helicase
Hydrolase
Magnesium
Manganese
Metal-binding
Nuclease
Nucleotide-binding
Phosphoprotein
RNA-binding
RNA-mediated gene silencing
Reference proteome
Repeat - Interacts With:
- P0C205; P55265-1; P55265-5; Q9UL18; Q9UKV8; Q8CJG0; Q96C10; P19525; Q96J94; O75569; Q15633
Publication
- PubMed ID:
- 10786632 20615407 10231032 12168954 14702039 12508121 15489334 10051563 15242644 14749716 16271387 16289642 16142218 16357216 15973356 16424907 17452327 17531811 17507929 18690212 18669648 18178619 19413330 19219043 21269460 21882293 21406692 23063121 22187960 24275569 17920623 19556464 21205968