Names & Taxonomy
- Uniprot ID:
- Q9NYA1
- Entry Name:
- SPHK1_HUMAN
- Status:
- reviewed
- Protein Names:
- Sphingosine kinase 1 (SK 1) (SPK 1) (EC 2.7.1.91)
- Gene Names:
- SPHK1 SPHK SPK
- Gene Names Primary:
- SPHK1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 384
- Sequence:
- MDPAGGPRGVLPRPCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm. Nucleus. Cell membrane. Note=Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.
Function
- Function:
- Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
- Catalytic Activity:
- ATP + sphinganine = ADP + sphinganine 1-phosphate.; ATP + sphingosine = ADP + sphingosine 1-phosphate.
- Cofactor:
- COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=14 uM for sphingosine
- Active Site:
- ACT_SITE 81 81 Proton donor/acceptor.
- Cross Reference Drug Bank:
- DB08868
- Gene Ontology Go:
- axon
cytoplasm
cytosol
nucleus
plasma membrane
synaptic vesicle
ATP binding
calmodulin binding
D-erythro-sphingosine kinase activity
DNA binding
magnesium ion binding
protein phosphatase 2A binding
sphinganine kinase activity
sphingosine-1-phosphate receptor activity
'de novo' posttranslational protein folding
blood vessel development
brain development
calcium-mediated signaling
cellular protein metabolic process
cellular response to growth factor stimulus
cellular response to hydrogen peroxide
cellular response to starvation
cyclooxygenase pathway
female pregnancy
inflammatory response
intracellular signal transduction
lipid phosphorylation
negative regulation of apoptotic process
positive regulation of angiogenesis
positive regulation of cell growth
positive regulation of cell migration
positive regulation of fibroblast proliferation
positive regulation of mitotic cell cycle
positive regulation of neuron projection development
positive regulation of neurotransmitter secretion
positive regulation of NF-kappaB import into nucleus
positive regulation of NF-kappaB transcription factor activity
positive regulation of peptidyl-threonine phosphorylation
positive regulation of protein ubiquitination
positive regulation of smooth muscle contraction
protein folding
regulation of interleukin-1 beta production
regulation of tumor necrosis factor-mediated signaling pathway
response to amine
response to ATP
response to interleukin-1
response to magnesium ion
response to progesterone
signal transduction
small molecule metabolic process
sphingoid catabolic process
sphingolipid biosynthetic process
sphingolipid metabolic process
sphingosine biosynthetic process
sphingosine metabolic process
sphingosine-1-phosphate signaling pathway
vascular endothelial growth factor receptor signaling pathway - Gene Ontology Biological Process:
- 'de novo' posttranslational protein folding
blood vessel development
brain development
calcium-mediated signaling
cellular protein metabolic process
cellular response to growth factor stimulus
cellular response to hydrogen peroxide
cellular response to starvation
cyclooxygenase pathway
female pregnancy
inflammatory response
intracellular signal transduction
lipid phosphorylation
negative regulation of apoptotic process
positive regulation of angiogenesis
positive regulation of cell growth
positive regulation of cell migration
positive regulation of fibroblast proliferation
positive regulation of mitotic cell cycle
positive regulation of neuron projection development
positive regulation of neurotransmitter secretion
positive regulation of NF-kappaB import into nucleus
positive regulation of NF-kappaB transcription factor activity
positive regulation of peptidyl-threonine phosphorylation
positive regulation of protein ubiquitination
positive regulation of smooth muscle contraction
protein folding
regulation of interleukin-1 beta production
regulation of tumor necrosis factor-mediated signaling pathway
response to amine
response to ATP
response to interleukin-1
response to magnesium ion
response to progesterone
signal transduction
small molecule metabolic process
sphingoid catabolic process
sphingolipid biosynthetic process
sphingolipid metabolic process
sphingosine-1-phosphate signaling pathway
sphingosine biosynthetic process
sphingosine metabolic process
vascular endothelial growth factor receptor signaling pathway - Gene Ontology Molecular Function:
- ATP binding
calmodulin binding
D-erythro-sphingosine kinase activity
DNA binding
magnesium ion binding
protein phosphatase 2A binding
sphinganine kinase activity
sphingosine-1-phosphate receptor activity - Gene Ontology Cellular Component:
- axon
cytoplasm
cytosol
nucleus
plasma membrane
synaptic vesicle - Keywords:
- 3D-structure
ATP-binding
Alternative splicing
Calmodulin-binding
Cell membrane
Complete proteome
Cytoplasm
Kinase
Membrane
Nucleotide-binding
Nucleus
Phosphoprotein
Polymorphism
Reference proteome
Transferase - Interacts With:
- P07858; P68104; Q14192; Q2M3C7; Q9Y4K3