Names & Taxonomy
- Uniprot ID:
- Q99873
- Entry Name:
- ANM1_HUMAN
- Status:
- reviewed
- Protein Names:
- Protein arginine N-methyltransferase 1 (EC 2.1.1.319) (Histone-arginine N-methyltransferase PRMT1) (Interferon receptor 1-bound protein 4)
- Gene Names:
- PRMT1 HMT2 HRMT1L2 IR1B4
- Gene Names Primary:
- PRMT1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 361
- Sequence:
- MENFVATLANGMSLQPPLEEVSCGQAESSEKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFKDKVVLDVGSGTGILCMFAAKAGARKVIGIECSSISDYAVKIVKANKLDHVVTIIKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVLYARDKWLAPDGLIFPDRATLYVTAIEDRQYKDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVTNACLIKEVDIYTVKVEDLTFTSPFCLQVKRNDYVHALVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTGEEIFGTIGMRPNAKNNRDLDFTIDLDFKGQLCELSCSTDYRMR
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Nucleus
Function
- Function:
- Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).
- Catalytic Activity:
- 2 S-adenosyl-L-methionine + -L-arginine = 2 S-adenosyl-L-homocysteine + -N(omega),N(omega)-dimethyl-L-arginine.
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1 uM for AdoMet
- Enzyme Regulation:
- ENZYME REGULATION: By BTG1, BTG2 and ILF3.
- Active Site:
- ACT_SITE 152 152
- Gene Ontology Go:
- cytoplasm
cytosol
methylosome
nucleoplasm
nucleus
enzyme binding
histone methyltransferase activity
histone methyltransferase activity (H4-R3 specific)
identical protein binding
methyl-CpG binding
methyltransferase activity
mitogen-activated protein kinase p38 binding
N-methyltransferase activity
poly(A) RNA binding
protein methyltransferase activity
protein-arginine N-methyltransferase activity
protein-arginine omega-N asymmetric methyltransferase activity
cell surface receptor signaling pathway
chromatin organization
histone H4-R3 methylation
histone methylation
negative regulation of megakaryocyte differentiation
neuron projection development
peptidyl-arginine methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
peptidyl-arginine N-methylation
positive regulation of erythrocyte differentiation
positive regulation of hemoglobin biosynthetic process
positive regulation of p38MAPK cascade
protein methylation
regulation of transcription, DNA-templated - Gene Ontology Biological Process:
- cell surface receptor signaling pathway
chromatin organization
histone H4-R3 methylation
histone methylation
negative regulation of megakaryocyte differentiation
neuron projection development
peptidyl-arginine methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
peptidyl-arginine N-methylation
positive regulation of erythrocyte differentiation
positive regulation of hemoglobin biosynthetic process
positive regulation of p38MAPK cascade
protein methylation
regulation of transcription, DNA-templated - Gene Ontology Molecular Function:
- enzyme binding
histone methyltransferase activity
histone methyltransferase activity (H4-R3 specific)
identical protein binding
methyl-CpG binding
methyltransferase activity
mitogen-activated protein kinase p38 binding
N-methyltransferase activity
poly(A) RNA binding
protein-arginine N-methyltransferase activity
protein-arginine omega-N asymmetric methyltransferase activity
protein methyltransferase activity - Gene Ontology Cellular Component:
- cytoplasm
cytosol
methylosome
nucleoplasm
nucleus - Keywords:
- Alternative splicing
Complete proteome
Cytoplasm
Methyltransferase
Nucleus
Polymorphism
Reference proteome
S-adenosyl-L-methionine
Transferase - Interacts With:
- Itself; Q9Y3Y2; Q5TAQ9; Q08211; Q01844; Q8IZU1; P04591; Q5JVS0; P61978; O43390; Q12906; P48552; Q8IZS5; Q9NR22; Q9NR22-2; Q14524; Q15772; Q96BD6; Q99619; O60506; P40337; Q96HA8