Names & Taxonomy
- Uniprot ID:
- Q7L266
- Entry Name:
- ASGL1_HUMAN
- Status:
- reviewed
- Protein Names:
- Isoaspartyl peptidase/L-asparaginase (EC 3.4.19.5) (EC 3.5.1.1) (Asparaginase-like protein 1) (Beta-aspartyl-peptidase) (Isoaspartyl dipeptidase) (L-asparagine amidohydrolase) [Cleaved into: Isoaspartyl peptidase/L-asparaginase alpha chain; Isoaspartyl peptidase/L-asparaginase beta chain]
- Gene Names:
- ASRGL1 ALP CRASH
- Gene Names Primary:
- ASRGL1
- Organism:
- Homo sapiens (Human)
Structure
- Length:
- 308
- Sequence:
- MNPIVVVHGGGAGPISKDRKERVHQGMVRAATVGYGILREGGSAVDAVEGAVVALEDDPEFNAGCGSVLNTNGEVEMDASIMDGKDLSAGAVSAVQCIANPIKLARLVMEKTPHCFLTDQGAAQFAAAMGVPEIPGEKLVTERNKKRLEKEKHEKGAQKTDCQKNLGTVGAVALDCKGNVAYATSTGGIVNKMVGRVGDSPCLGAGGYADNDIGAVSTTGHGESILKVNLARLTLFHIEQGKTVEEAADLSLGYMKSRVKGLGGLIVVSKTGDWVAKWTSTSMPWAAAKDGKLHFGIDPDDTTITDLP
- Proteomes:
- UP000005640
Subcellular location
- Subcellular Location:
- Cytoplasm
Function
- Function:
- Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic activity toward beta-aspartyl dipeptides and their methyl esters, including beta-L-Asp-L-Phe, beta-L-Asp-L-Phe methyl ester (aspartame), beta-L-Asp-L-Ala, beta-L-Asp-L-Leu and beta-L-Asp-L-Lys. Does not have aspartylglucosaminidase activity and is inactive toward GlcNAc-L-Asn. Likewise, has no activity toward glutamine.
- Catalytic Activity:
- L-asparagine + H(2)O = L-aspartate + NH(3).
- Kinetics:
- BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.4 mM for L-asparagine (L-Asn)
- Enzyme Regulation:
- ENZYME REGULATION: Glycine accelerates autocleavage into an alpha and beta chain.
- Active Site:
- ACT_SITE 168 168 Nucleophile.
- Cross Reference Drug Bank:
- DB00174 DB00128
- Gene Ontology Go:
- cytoplasm
cytosol
nucleus
asparaginase activity
beta-aspartyl-peptidase activity
asparagine catabolic process via L-aspartate
cellular nitrogen compound metabolic process
L-phenylalanine catabolic process
small molecule metabolic process - Gene Ontology Biological Process:
- asparagine catabolic process via L-aspartate
cellular nitrogen compound metabolic process
L-phenylalanine catabolic process
small molecule metabolic process - Gene Ontology Molecular Function:
- asparaginase activity
beta-aspartyl-peptidase activity - Gene Ontology Cellular Component:
- cytoplasm
cytosol
nucleus - Keywords:
- 3D-structure
Acetylation
Alternative splicing
Autocatalytic cleavage
Complete proteome
Cytoplasm
Hydrolase
Protease
Reference proteome